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Scientist, Discovery Research
Nohla Therapeutics in Seattle, Washington
 
 
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Date Posted July 11, 2018
Category
Science-Pharmacology
Employment Type Fulltime
Application Deadline Open until filled
 
 
 

As a member of the Research group, this position will contribute to the generation of new off-the-shelf stem cell therapies that fuel the research pipeline. The Scientist will perform hands-on laboratory work associated with the isolation of stem cells from cord blood, developing new methods for cell culture expansion of stem cells, and developing immune assays for evaluating cellular-therapy products. This person will work closely with the research team to collaborate on projects as well as work independently to achieve the overall corporate objectives. The position will report to the Senior Director of Discovery Research.

Responsibilities

The position will have responsibilities that include: 

  • Design, plan, execute and interpret experiments to generate data for the development of novel cellular therapies

  • Develop and refine immune assays and models, both in vitro and in vivo, for evaluating cellular-therapy products

  • Develop new methods to expand stem cells from human cord blood and peripheral blood

  • May carry out gene editing of stem cells using a variety of gene-editing technologies

  • Analyze data using software including FlowJo, Excel, and GraphPad Prism; prepare and present data to various groups within the organization and externally as needed

  • Perform routine cell culture, isolation of stem cells from cord blood, peripheral blood, and mouse bone marrow and expansion of stem cells using standard protocols

  • Provide critical lab support while working with other scientists in a collaborative team environment

  • Bring forth new ideas from literature

  • Work closely with the research team to interpret data and guide the research discovery pipeline

  • Assist in managing external, collaborative projects as directed by senior management

  • Contribute to the preparation of patents and manuscripts

  • Travel as needed to coordinate/liaise with external collaborators

  • May supervise associate staff

  • Maintain an accurate laboratory notebook, manage electronic data, and comply with laboratory safety procedures.

  • Mentors and inspires team members as a tireless champion of bringing lifesaving cellular therapies to people in need

 

Qualifications

 The ideal candidate will have qualifications and experience that include: 

  • BA/BS or MS with 10+ years’ experience or PhD with 2-4+ years’ experience in the biotechnology or pharmaceutical industry

  • Experience in the isolation of stem cells from human cord blood, peripheral blood and mouse bone marrow

  • Extensive hands-on experience in primary cell culture

  • Experience in optimization of stem cell expansion conditions

  • Hands-on expertise in methods to isolate and characterize cells including flow cytometry sorting and analysis, immune cell-based assays (activation, suppression, proliferation, cytokine analysis) and molecular/biochemical assays (RNA/DNA isolation, qPCR, ELISA, western, microscopy) using primary cell culture, patient samples, and mouse models

  • Ability to independently design, execute, interpret and communicate experimental results

  • Ability to think critically and demonstrate troubleshooting and problem-solving skills

  • Attention to detail and ability to organize and prioritize tasks in a timely and accurate manner

  • Comfortable in a fast-paced small company environment with minimal direction and able to adjust workload based upon changing priorities; ability to work independently and within a team; have a “do-what-it-takes” attitude

  • Proficient in MS Office Suite, Flow cytometry data analysis, GraphPad Prism, and other applications

  • Excellent interpersonal, verbal, and written communication skills and record-keeping ability

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